Answer to Question #204600 in Biochemistry for mbali

Question

Discuss the principles of ion exchange and affinity chromatography

Answer 1

The anion exchange chromatography separates anions. Affinity chromatography is a separation technique based on the specific interaction between an immobilized ligand, and it's binding protein. For example, nickel which has a high affinity to histidine, can be used to separate histidine from a mixture of other protein.