Answer to Question #218564 in Biochemistry for miss Claire Yeboah

Question

The student obtains the following results: it took 1475 seconds for the disappearance of starch when the 0.05% amylase was used, 12 minutes and 15 seconds for the 0.1% amylase, whilst the 0.15% amylase took 520 seconds. The 0.25% amylase gave a result of four minutes and 35 seconds, whilst the 0.2% amylase took 6 minutes and five seconds.

The student is informed that as the results reflect the use of the disappearance of substrate and not the production of product, their results must be converted to 1/T (where T=time in seconds) for the rate of reaction.

Answer 1

Let us design a table with these results and using 1/T instead of T:

Now the graph from that table:

The higher the NaCl concentration, the higher the reaction rate for the enzyme amylase. This occurs because Cl- ions work as an activator to amylase; it binds to allosteric sites and increases their activity