Answer to Question #294144 in Biochemistry for Ben

Question

What is the role of sodium dodecyl sulfate (SDS) in the isolation of plasmid DNA (select one) 

a. helps dissolve DNA 

b. binds to DNA and despiralizes it 

c. binds divalent ions and inhibits DNA 

d. precipitates RNA 

e. denatures cellular proteins and helps to lyse the cell membrane 

Answer 1

Given its chemical properties, SDS is an anionic detergent that denatures secondary and nondisulfide-linked tertiary protein structure, shattering the native shape. SDS provides a negative charge to each protein as a function of their size. Furthermore, SDS can be used to aid in lysing cell during DNA extraction.

In conclusion, the right answer is

e. denatures cellular proteins and helps to lyse the cell membrane