Answer to Question #331986 in Biochemistry for Eman

Question

Write the function of each component of TBE and TAE buffer along with individual molarity from given mass

T= Tris

A= Glacial acetic acid

E= EDTA

B= Boric Acid

10x TAE Recipe

For 1L of 10x solution,

• 48.5 g tris

• 11.4 mL glacial acetic acid

• 20 mL 0.5M EDTA (pH 8.0)

TBE Buffer 10x Stock Recipe

• 108 g tris base

• 55 g boric acid

• 900 ml double-distilled H2O

• 40 ml 0.5 M EDTA solution (pH 8.0)

Adjust volume to 1 L.