Biochemistry Answers

Tendency of the solute to spread throughout the solution until the composition is homogeneous

Suggest a pH of a buffer that can be used in an electrophoresis experiment to ensure that the amino acid asparagine will migrate to the cathode portion of the matrix. Explain your answer briefly.

Predict the migration of the amino acid tryptophan in an electrophoresis matrix using buffer at pH 2, 7, and 10. Illustrate it by placing a spot indicating the amino acid at each electrophoresis diagram below labeled with the corresponding pH. Show the ionization of tryptophan and the calculation of its pI below.

Explain how light energy is funneled from the antenna to the special pair resulting in electric charge separation at the reaction centre chlorophyll.

Explain the roles of the various pigments during the light reactions

What is HDL

Given that a solution containing a 0.2 M concentration of a weak acid and 0.5 M of the negatively charged ion has a pH value of 4.4, find the pK value of the weak acid. Express the result to two decimal places.

draw the his- ala- lys-glu-gly structure at pH 7

The velocity of an enzyme-catalyzed reaction that follows Michaelis-Menten kinetics was

measured at several substrate concentrations (shown below).

i. Calculate 𝐾M and V𝑚𝑎𝑥 from the data given.

ii. Determine KM and Vmax

iii. The second set of velocities represent the rate of the reaction when an inhibitor is

added. Plot these on the same graph as the first and determine KM and Vmax

iv. What type of inhibitor is it?

[S](M) V0 (μM/min) V(+ inhibitor)

0.00006 6.66 4

0.0001. 9.30 3

0.002. 15 6

0.006 22.03 8

0.08 29 9

0.2. 35.6 10.6

With help of diagram explain the pentose phosphate pathway or hexose monophosphate shunt,