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a large capacity bench top preparative centrifuge works in the rcf range of _______ while a high speed refrigerated centrifusion up to ____


____ of all diatomaceous supports in glc is necessary which is done by ____


Would the use of pH buffers help prevent muscle fatigue during exercise? 

What happens to amylose starch that is not fully hydrolyzed in the small intestine? What might be the clinical consequence? 

 


Why sweating in effective way to cold the body


Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as

b-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in

Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-

ml solution containing 10-9 g of purified penicillinase was measured as a function of the

concentration of penicillin. Assume that the concentration of penicillin does not change

appreciablyduring the assay.

(a) Plot V0 versus [S] and 1/V0 versus 1/[S] for these data. Does penicillinase appear to obey

Michaelis-Mentenkinetics? If so, what is the value of KM?

(b) What is the valueof V max?

(c) What is the turnover number of penicillinase under these experimental conditions?

Assume one active site per enzyme molecule


The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-


chains and two β-chains tightly bound.


a) Calculate the number of amino acids present in the α-chains


b) What is the length of each β-chain?


c) What is the sum of the length of the two α-chains?


d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha

The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-



chains and two β-chains tightly bound.



a) Calculate the number of amino acids present in the α-chains



b) What is the length of each β-chain?



c) What is the sum of the length of the two α-chains?



d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha

Study the polypeptide structuresequence of a β-strand shown below


b)Calculate the weight of the protein in KDa


Lys-Gly-Asp-Trp-Ser-Glu


a)Draw the chemical structure of the polypeptide. Indicate on the structure the


backbone and peptide bonds


c)Calculate the length of the protein.

.The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α


chains andtwo β-chains tightly bound.


d)Find the length of the tetrameric proteinhemoglobinassuming its all an alpha


a)Calculate the number of amino acids present in the αchains


b)What is the length of each β-chain?


c)What is thesum of thelengthof the two α-chains?


helical structure.

Draw the chemical structure of cholesterol and state its functions


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