a large capacity bench top preparative centrifuge works in the rcf range of _______ while a high speed refrigerated centrifusion up to ____
____ of all diatomaceous supports in glc is necessary which is done by ____
Would the use of pH buffers help prevent muscle fatigue during exercise?
What happens to amylose starch that is not fully hydrolyzed in the small intestine? What might be the clinical consequence?
Why sweating in effective way to cold the body
Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as
b-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in
Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-
ml solution containing 10-9 g of purified penicillinase was measured as a function of the
concentration of penicillin. Assume that the concentration of penicillin does not change
appreciablyduring the assay.
(a) Plot V0 versus [S] and 1/V0 versus 1/[S] for these data. Does penicillinase appear to obey
Michaelis-Mentenkinetics? If so, what is the value of KM?
(b) What is the valueof V max?
(c) What is the turnover number of penicillinase under these experimental conditions?
Assume one active site per enzyme molecule
The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-
chains and two β-chains tightly bound.
a) Calculate the number of amino acids present in the α-chains
b) What is the length of each β-chain?
c) What is the sum of the length of the two α-chains?
d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha
The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-
chains and two β-chains tightly bound.
a) Calculate the number of amino acids present in the α-chains
b) What is the length of each β-chain?
c) What is the sum of the length of the two α-chains?
d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha
Study the polypeptide structuresequence of a β-strand shown below
b)Calculate the weight of the protein in KDa
Lys-Gly-Asp-Trp-Ser-Glu
a)Draw the chemical structure of the polypeptide. Indicate on the structure the
backbone and peptide bonds
c)Calculate the length of the protein.
.The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α
chains andtwo β-chains tightly bound.
d)Find the length of the tetrameric proteinhemoglobinassuming its all an alpha
a)Calculate the number of amino acids present in the αchains
b)What is the length of each β-chain?
c)What is thesum of thelengthof the two α-chains?
helical structure.
Draw the chemical structure of cholesterol and state its functions