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Discuss the haworth and fischer projection structure of glucose and fructose.


Calculate the isoelectric point of glutamic acid .


Suggest why blinking of the eyes is coordinated by nerves and not hormones.



what is the role of starch in the titration?



The ability of hemoglobin (Hb) to carry oxygen throughout the body as oxyhemoglobin (HbO2) is dependent on the pH of the blood. What effect would acidosis have on the ability of a patient to transport oxygen?


Why is the PI of human insulin higher than PI of duck insulin


What would happen to an organism that lacked the enzyme that catalyzes the first step in glycolysis? 


Calpain 1 is a cysteine-protease existing as a heterodimer made up of a large and small subunit. The large subunit is 714 residues long (mass 81.9 kDa) with a pI of 5.49. The small subunit is 268 residues long (mass 28.3 kDa) with a pI of 5.05. Calpain 1 is inhibited by calpastatin, an intrinsically disordered protein of 708 residues (mass 76.5 kDa) with a pI of 4.97. Suggest a purification method that would allow you to separate calpain 1 from calpastatin. Describe conditions and explain why the chosen method would work and give reasons why it wouldn’t work.


Write a short note on Dietary trace elements.


Illustrate the titration curve of lysine showing the cation, zwitterion and anion on the curve
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