Answer to Question #195317 in Bioinformatics for Faye

Question

1. For each seq:

a) Identify the protein and species

b) At approx what amino acid number is the most hydrophilic region

of the protein?

c) Do you expect it to be secreted? If so, by what mechanism?

d) List the motifs that are identified. Use the standard parameters for the tool

you choose to use.

Seq1

MQVNTFSNIASMARTQVSNKKADDAKENTKDKNVQSANSSKDVDKNTLEKLNALG

GKGITQIYLVQFQQQTMNAVIGSSNAQTGLDSLLNGANLDTAKSILTNIDFASLGYSS

KNPLDMNTDELQQLVSEDGFFGVENTANRIADFVIKGGGDDVEKLKKGLEGMKKG

FEQAEKMWGGELPQISQNTIDAALKKVSDRIDELGGKTLDLQA

Seq2

MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTR

REAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Seq3

MRLVILTLLIVGVFASNDDLWHQWKRIYNKEYKGADDDHRRNIWEQNVKHIQEHNL

RHDLGLVTYKLGLNQFTDMTFEEFKAKYLTEMPRASELLSHGIPYKANKRAVPDRID

WRESGYVTEVKDQGGCGSCWAFSTTGAMEGQYMKNEKTSISFSEQQLVDCSGP

FGNYGCNGGLMENAYEYLKRFGLETESSYPYRAVEGQCRYNEQLGVAKVTGYYT

VHSGDEVELQNLVGCRRPAAVALDVESDFMMYRSGIYQSQTCSPDRLNHGVLAV

GYGIQDGTDYWIVKNSWGTWWGEDGYIRMVRKRGNMCGIASLASVPMVAQFP

Answer 1

Quaternary protein & Structural specie

Because the charged and polar amino acids are hydrophilic, they are usually found at the surface of a water-soluble protein, where they not only contribute to the solubility of the protein in water but also form binding sites for charged molecules.

No

The TATA promoter sequence is an example of a highly conserved DNA sequence motif found in eukaryotes. Another example of motifs: binding sites for transcription factors (TF) near promoter regions of genes, etc.