Bioinformatics Answers

a. In the agarose gel electrophoresis practical class, you are instructed to prepare a 750 ml of 1X TAE buffer from 25X TAE stock buffer. Subsequently, you are requested to prepare 35 ml of 0.75% (w/v) agarose gel using 1X TAE buffer that you prepared . Show your calculation and describe how you prepare the agarose gel

Without a bioinformatics tool, how can you calculate the molecular weight of protein?

using python
i need to parse out from below EMBL file! i Just need to parse out the sequence ID (line
starts with ID), description (DE) and sequence (SQ). Use at least one function.

Below is EMBL file:

ID M91373; SV 1; linear; mRNA; STD; PLN; 1131 BP.
XX
AC M91373;
XX
DT 24-APR-1992 (Rel. 31, Created)
DT 17-APR-2015 (Rel. 83, Last updated, Version 4)
XX
DE Cucumis sativus peroxidase mRNA, complete cds.
XX
KW peroxidase.
XX
OS Cucumis sativus (cucumber)
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids;
OC fabids; Cucurbitales; Cucurbitaceae; Cucumis.

Write python program to parse out embl id

You are provided with the following amino acid sequence:

1 mfkemrlkkr emtkedtvev lkngefgtfs tisengypyg vavnyvyfnd siyfhcarng
61 hkldnisknn kvsflvvane svipdkfstt yssaivfgka ctveneekkn alveiikkys
121 kgffeegmky iekdmnlttv vkieidhisg kasrl

Use online bioinformatics tools, and search the scientific literature to answer the following questions

1. What is this protein? (name, function from blast search)
3. Can you make a prediction of its function? What evidence supports this prediction?
5. If the protein occurs in other organisms, can this information be used to glean further, more specific information on the function of the protein?
6. How similar is your query sequence to other homologous sequences – present this information as an alignment of relevant sequences, and use these sequences to construct a phylogenetic tree (use Clustal omega).

Give the full account of Gene prediction method

it is noticed that major sequence alignments differ in approach computational complexity and accuracy. Do you agree with this? Explain it with examples

Identify 5 environmental factors that may influence communication

Discuss 6 point that you need to consider in order to improve your communication