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I'm trying to localise a protein called "Protein B."


I've made a vector which encodes protein B that is tagged with an epitope derived from the flu virus. This vector is then transformed into a human cell line, and antibodies to the flu virus will be used to locate the protein.


2 staining procedures are done, one with no alcohol, and one with 2% (v/v) alcohol.


In the staining with no alcohol, you can see uniform staining of the area outside the nucleus.


In the staining with 2% alcohol, you see speckled staining in the area outside the nucleus.


I know that protein B is located in the mitochondria/cytoplasm.


My question is: What does the uniform staining vs speckled staining indicate? Also, why does this type of staining occur?


How can active transport occur in red blood cells even though they lack mitochondria?


How diffusion of solid particles can take place within a cell membrane?


In an experiment, a scientist decides to study the effect of exercise on cholesterol levels in people. He studies two set of people—those who exercise every day for an hour and those who don’t exercise at all. In this case, the statement that people who exercise for an hour may have lower cholesterol levels is____. To test this statement, the scientist would measure cholesterol levels in exercisers and non-exercisers. The cholesterol levels would be___.


Why do we need to know how plants reproduce

How can active transport occur in mitochondria even if they lack mitochondria?


If the low FOV= 2mm, low magnification is 4x, medium magnification is 10x and high magnification is 40x, calculate the high FOV


Which of the following is an example of active transport


The green pigment in plant cells is called?


Task 2. Draw a mitotic cycle, indicate mitogenic stimuli and inhibitors that determine different phases of the cell cycle 


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