Answer to Question #197042 in Genetics for Kirsten

Question

Assume that the supplier of your primer set informs you that they delivered the wrong forward primer to you. The forward primer sent you is for a gene in another species, with no annealing sites in the human genome. What would your expected result be if you set up your PCR reaction mix using this primer set? Motivate your answer.

Answer 1

Modified PCR Setups- For complex primer and template sequences, consider either a hot start or touchdown PCR setup.