Answer to Question #23855 in Microbiology for mojtaba

Question

which culture media is suitable for growing S.mutans ?
we used muller hinton agar.but we want to culture it in an overnight.
thank you so much.

Answer 1

Cultural environment streptococci blood agar - used for isolation of beta-hemolytic streptococci group A medium enriched with ribonucleic acid, maltose, helps to increase the size of the colonies and the best clarity and sharpness of hemolytic zones produced by these organisms.

The technique of using Selective streptococcal agar made from beef extract and casein peptone in which relatively little dextrose, which allows you to add the blood of animals to determine the hemolytic activity. To cultivate the sample, make planting flora, causing a smear on the third of the surface of agar, and make seed for the remainder of the stroke to obtain isolated colonies. Material not cultured with swabs can be planted on a medium with a sterile bacteriological loop. Bar seeding technique is mainly used to obtain isolated colonies from specimens containing mixed flora. Raise the plate upside down (agar side up) at 35-37 ° C for 18-48 hours in an enriched CO2 atmosphere.