Answer to Question #331295 in Microbiology for sofie

Question

1.   Give two factors that may affect a good gram staining technique output. Provide an explanation of how these factors influence the result. 

Answer 1

The most common reasons for false gram reaction.

Some bacterial species tend towards gram variable, and will show both colors although most often gram positive.

Using old cultures (preferably, the cultures should be 18-48 hours old).