Answer to Question #105639 in Molecular Biology for Kelly

Question

What Tm value (to 1 decimal place) would you use for the forward primer when calculating the annealing temperature for the last 25 cycles of PCR? Assume the forward primer sequence is 5’ CAT ATG GGC GTT ACA GGA ATA TTG 3’.

Do show your calculations, provide the answer with units if possible.

Answer 1

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