Answer to Question #64271 in Molecular Biology for Sanjukta Ghosh

Molecular Biology

Question #64271

How does RNA polymerase determine which one is the template strand?

Expert's answer

The RNA polymerase itself does not differentiate between two strands of the DNA. Instead, it is guided by the orientation of initiatory complex (composed of transcription factors). Initiatory transcription factors can recognize specific nucleotide sequences on the promoter, therefore, these proteins choose the template strand.

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22.12.16, 17:48

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Sanjukta Ghosh

21.12.16, 22:27

In case of prokaryotic RNAP is it sigma factor that specifically binds to -10 and -35 sequences of template strand(promoter)?

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