Molecular Biology Answers

1. if you want to overexpress a protein in ecoli. The protein is from human ocular cells. What vector would you use to express the protein?. You know the sequence of the gene for the ocular protein, where would you get the DNA you need to clone into the vector? And what regulatory sequences need to be present in your vector for expression to occur?
2. how will u make a conditional mutant in mice using cre/lox system?

thank you

I want to know the general concentration of catalase in Brócoli, potatoes, onions, carrots and cucumbers

Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.

Given the two amino acids below, perform a pairwise alignment using the FASTA method. 

Sequence1  AKDMTGPNL

Sequence2 LDMTGPNAK

In the hashing table what is the offset value for Leucine?

Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.

How can many different DNA molecules be made up of only four bases?

If the transcription factor is reduce. Can the hypothesis be solve this issue by upregulating TF

If by mutation or aging if the transcription factor diminish.....then is there a way to upregulate the transcription factor

Only one strand of the double helix is used as template for transcription. How would the transcription machinery recognize the template strand for transcription of a particular gene? Is it necessary that this strand will be used as template for transcription of all genes of that particular organism?

Suppose we have a genomic DNA of 1000 kb. It gives two products: 1000 kb product and 200 kb product. What can you infer about the nucleic acid nature of each product? Which process will give the 1000 kb product and which will generate the 200 kb product?