Molecular Biology Answers

Why is the generation of single and double mutants were done using PCR-based QuikChnage site-directed mutagenesis?

How would you verify that the plasmid contains your insert of interest, in the proper position and orientation?

Describe how you would calculate ∆G0′ for the reaction: glucose + 6 O2 S6 CO2 + 6 H2O. If you were told that this reaction is highly exergonic, what would be the arithmetic sign (negative or positive) of the ∆G0′ you would expect for this reaction?

Questions 

Answer the following questions at the end of the report: 

1. Give ONE example each for commercial kits that can be used to extract i) genomic DNA from blood and ii) plasmid DNA from bacteria. Provide the full names of the kits and URLs to the products. 

2. Why vigorous mixing at step 4 of part C in the practical handout must be avoided. 

3. Besides agarose gel electrophohoresis, name another method to check the quantity and quality of purified DNA samples. 

C. Preparation of plasmid DNA from bacteria cell (E. Coli) – Alkaline method.

4. Add 200 µL of Alkaline lysis solution II (0.2 N NaOH, 1% SDS) to the suspension and mixed by inverting the tube 5 – 8 times until the suspension become clear and sticky.

In a laboratory lesson in histology, a student studied a micropreparation at a low magnification of the microscope, and then wanted to examine the structures of interest at a high magnification, but, despite attempts to focus the image, he did not achieve clarity, and the glass of the specimen broke. What mistakes were made when studying the micropreparation?

If you will construct several phylogenetic trees of mammalian species using protein sequences from cytochrome c, hemoglobin, and fibrinopeptides which trees would have a more or less similar tree topology? Which trees would look very different from each other? Why is this so?

You are starting your own lab and have been given open-ended funding from a

generous donor to pursue advanced "-omics" approaches to completely understand the

influence of environment on growth and development of Plasmopara obducens (powdery

mildew) on Impatiens walleriana.

Assume the following:

There are no advanced "-omics" data on either the pathogen or the host.

You can grow the pathogen on defined media in a lab.

You can readily infect the host with the pathogen.

Environmental factors are known to influence disease severity and/or progression.

Your task is to develop a research program that will identify molecular targets for

combatting this pathogen using advanced "-omics" approaches. Be as broad as

possible and keep in mind the multiple levels that constitute gene expression regulation.

You must also consider experimental design and data analysis (and confirmation of

results if appropriate). 

: What makes ATP essential to the organelles and the cell itself?