Answer to Question #212062 in Biology for Phareedadiva

Question

The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS.

Combine the following:

• 80g NaCl
•  2g KCl
•  14.4g Na₂HPO₄ (dibasic anhydrous)
•  2.4g KH₂PO₄ (monobasic anhydrous)
• 800mL distilled H₂O 

1. Adjust pH to 7.4 with HCl
2. Add H₂O to 1L
3. Autoclave for 20 minutes on liquid cycle. Store at room temperature.

Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?

Answer 1

Phosphate-buffered saline (PBS) solution is an isotonic buffer (pH 7.4) that is commonly used in experiments involving biological specimens. This is because it mimics the pH, osmolarity, and ion concentrations in the human body, preventing denaturation or degradation of the desired analytes.