Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as
b-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in
Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-
ml solution containing 10-9 g of purified penicillinase was measured as a function of the
concentration of penicillin. Assume that the concentration of penicillin does not change
appreciablyduring the assay.
(a) Plot V0 versus [S] and 1/V0 versus 1/[S] for these data. Does penicillinase appear to obey
Michaelis-Mentenkinetics? If so, what is the value of KM?
(b) What is the valueof V max?
(c) What is the turnover number of penicillinase under these experimental conditions?
Assume one active site per enzyme molecule
Give two reasons why the diagram above does NOT represent a desmosome.
State any six different mechanisms how some species decrease the chances of their
mate mating with another individual.
Discuss what happens when an individual with 17 chromosomes mates with an individual of the same species with 16 chromosomes.
Make use of a neat, annotated schematic representation to discuss the structure and
function of desmosomes.
Pharmaceutical and healthcare companies are very interested in substances that affect the mitochondria and associated energy releasing metabolic pathways. Depending on their effect, such substances may potentially be developed into ‘weight-loss’ drugs or ‘weight-gain’ drugs. Some substances are marketed as nutritional supplements that increase energy levels and enhance athletic performance.
The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-
chains and two β-chains tightly bound.
a) Calculate the number of amino acids present in the α-chains
b) What is the length of each β-chain?
c) What is the sum of the length of the two α-chains?
d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha
The human hemoglobin is a tetrameric protein of about 64.5 KDa, consisting of two α-
chains and two β-chains tightly bound.
a) Calculate the number of amino acids present in the α-chains
b) What is the length of each β-chain?
c) What is the sum of the length of the two α-chains?
d) Find the length of the tetrameric protein hemoglobin assuming its all an alpha
Briefly describe the parts of an unmyelinated and a myelinated neurone. State the functions of neurons and neuroglia. (10%- 5 marks for structure of neurones, 5 marks for functions)
Study the polypeptide structuresequence of a β-strand shown below
b)Calculate the weight of the protein in KDa
Lys-Gly-Asp-Trp-Ser-Glu
a)Draw the chemical structure of the polypeptide. Indicate on the structure the
backbone and peptide bonds
c)Calculate the length of the protein.