Answer to Question #273755 in Biochemistry for Karis

Question

A. Effect of temperature

Prepare 3 test tubes as follows

Test tube no. 1- 5ml 1% cooked starch solution + 1ml saliva. Keep in a water bath at 40°C

Test tube no. 2- 5ml 1% cooked starch solution+ 1ml saliva. Keep in a water bath at 60°C.

Test tube no. 3- 5ml 1% cooked starch solution+ 1ml saliva. Keep in a water bath at 10°C.

At 5 minutes, take a drop of the reaction mixture from test tube no. 1(stir the contents of the test tube before taking a drop) and with iodine solution. Perform the iodine test for a period of 1 hour. Tabulate your results.

Answer 1

Test tube no. 1 = Color of iodine persists

Test tube no. 2 = Test solution turns blue-black

Test tube no. 3 = Test solution turns blue-black