Answer to Question #337420 in Biochemistry for sana

Question #337420

- Briefly explain why we incubate the purified plasmid with the restriction enzymes, BamHI and XhoI, at exactly 37oC. 


1
Expert's answer
2022-05-05T17:31:03-0400

Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures



Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.


BamHI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

XhoI has 100% activity at 37°C, however there is significant increase of star activity when incubated overnight at 37°C


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