Biochemistry Answers

Blood sample is taken from 45 year old men after and overnight fast. Which will be at high concentration?

A. Alanine

B. Glucose

C. Glucagon

D. Ketine bodies

E. FFA

What are the advantages and disadvantage of molecular oxygen having high

affinity for electrons?

Ornithine transcarbamoylase deficiency can lead to the accumulation of what?

Provide a step-by-step diagram showing the β-oxidation of 2-methylhexanoic acid (see figure below). You only need to show the first cycle. Clearly indicate the products that form after the first cycle of β-oxidation.

You are a team of biochemists, botanists, and microbiologists who were tasked to isolate and identify antimicrobial peptides (AMPs) from endemic plants in the Philippines. In one of your studies, you were able to isolate 4 AMPs from a certain shrub in the mountainous area of Cebu, where 3 of them consists of amino acids only while the last one has a carbohydrate moiety. The AMPs were tested using amino acid analyzer and found out the following sequences for the 4 peptides

Amino Acid Sequence

1. Val-Gly-Leu-Phe-Asp-Phe-Val-Phe-Tyr-Gly-Ser-Phe (VGLFDFVFYGSF)
2. Asn-Pro-Cys-His-Glu-Ala-Cys-Cys-Ser-Cys-Leu-Cys (NPCHEACCSCLC)
3. Gly-Gly-Leu-Trp-His-Ala-Val-Trp-Thr-Trp-Phe-Ala-Trp (GGLWHAVWTWFAW)
4. (with carbohydrate moiety) Ser-Leu-Glu-Lys-Gly-Leu-Asp-Gly-Ala-Lys-Lys-Ala (SLEKGLDGAKKA)

Now create a procedure using different color reactions that would easily distinguish the four peptides from one another

II. Hypothesis-Testing Method Design

A. Problem

B. Theory

C. Hypothesis

D. Procedure

E. Results and Discussion

F. Conclusion

Predict the migration of the amino acid tryptophan in an electrophoresis matrix using buffer at pH 10. Illustrate it by placing a spot indicating the amino acid at each electrophoresis diagram below labeled with the corresponding pH. Show the ionization of tryptophan and the calculation of its pI below

Predict the migration of the amino acid tryptophan in an electrophoresis matrix using buffer at pH 7. Illustrate it by placing a spot indicating the amino acid at each electrophoresis diagram below labeled with the corresponding pH. Show the ionization of tryptophan and the calculation of its pI below

A sample containing mixture of methionine, proline, cysteine, histidine, and glutamic acid was subjected to electrophoresis at pH 4. Show the migration of each amino acid in the electrophoresis matrix by drawing spots corresponding to the location of each amino acid. Label each the spots with the 3-letter abbreviation of the amino acid. Show the calculation of pI of each amino acid below. The ionization of each amino acid is no longer necessary to be illustrated.

Illustrate below the structure of the peptide with the sequence WATERS

a. What is the amino acid residue at the C-terminal of the peptide? __________________

b. What is the amino acid residue at the N-terminal of the peptide? __________________

c. How many peptide bonds are present in the peptide? __________________

Name the following peptides with the following amino acid sequence:

 GSM

 CARE

 DREAMER

 LIPIDS

 VITAMINS