Biochemistry Answers

The use of broad-spectrum antibiotics can produce a bleeding diathesis characterized by hematomas, hematuria, melena, and bleeding from the gums by decreasing the normal gut flora and inducing a deficiency of a certain vitamin.

7.2.1 Which vitamin is deficient? Explain

Ketone bodies are exported from the liver for use by other tissues. Explain the enzymatic

properties of the liver that contributes to its special ability to export these compounds.

Discuss the principles of ion exchange and affinity chromatography

Draw the structure of the tripeptide Ser-His-Asn in the ionic form that predominates at

pH 7. Show all atoms (including hydrogens) as well as any charges as necessary.

Explain the mode of action of fluoride and bisulphate on the fermentation pathway. Predict their likely effect if the yeast treated with these agents had been respiring.

make a detailed schematic diagram of the classification of lipids classification by function classification by structure

make a detailed schematic diagram of the classification of lipids classification by function classification by structure

Adenosine triphosphate, a peptide (1 kDa) and Green Fluorescent Protein (27 kDa) were mixed in solution and analysed simultaneously using native mass spectrometry. Assume that the three components are all ionised equally and transmit equally into the mass spectrometer. Sketch what the mass spectrum of this mixture would look like, providing an explanation for your answer.

How deficiency of riboflavin affects other vitamins especially vitamin B

• 2) Why are the ratios of OD260/OD280 and OD260/OD280 for clean nucleic acids about 2.0? Show your answer by drawing and explaining a DNA absorbance spectrum from 200 to 300 nm. Which type of contaminations can you detect with these measurements?