Biochemistry Answers

1. Which of the following processes generates the most ATP? (Account for the no. of ATP)

a.) Citric Acid Cycle

b.) Fatty Acid oxidation

c.) Glycogenolysis

d.) Glycolysis

e.) Pentose phosphate pathway

Destroying the Trojan horse. Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10^-9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay.

analysis vitamin C in cabbage

For the purposes of the cell adhesion experiment, the cells are then diluted in media such that a 200 µl volume contains 9 x 103 cells. This volume is then added per well for the cell adhesion assay. In total, you will test 40 wells of endothelial cells from each genotype for each experimental condition (with or without TNFα).

1. Comment “Fatty acids are storage fuels”. The weight of a person ABC is 65 kg. About 11.5 kg of his weight is due to the fat deposits in the body. He went for a hunger strike. Can you explain how long he can survive? i. without water and food? ii. without food but with water? 

The weight of person ABC is 65 kg. About 11.5kg of his weight is due to fat deposits in his body. He went for a hunger strike. Can you explain how long he can survive?

1. Without food and water

2. With water and without food

Carbonic anhydrase is strongly inhibited by the drug acetazolamide, which is used as a diuretic. Carbonic anhydrase plays a significant role in the secretary processes because it participates in regulating the pH and bicarbonate of body fluids. The experimental curve of initial reaction velocity versus [S] for the carbonic anhydrase is shown below. When the experiment is repeated in the presence of acetazolamide, the lower curve is obtained. Determine the nature of inhibition. Explain with reason.

. A man ate food with low protein, high carbohydrate produces 6.2 g urea per day. The same man excreted 26.2 g urea per day after three days. Explain the data with logical reasoning.

. The weight of a person ABC is 65 kg. About 11.5 kg of his weight is due to the fat deposits in the body. He went for a hunger strike. Can you explain how long he can survive? i. without water and food?

The weight of person ABC is 65 kg. About 11.5kg of his weight is due to fat deposits in his body. He went for a hunger strike. Can you explain how long he can survive?

1. Without food and water

2. With water and without food