Biochemistry Answers

What Is The Absolute Difference In [H+] Between 2 Aqueous Solutions, One Of PH 2.0 And One Of PH 3.0? What is the [OH-] concentration of the solution of pH 2.0?

Comment “Fatty acids are storage fuels”. The weight of a person ABC is 65 kg. About 11.5 kg of his weight is due to the fat deposits in the body. He went for a hunger strike. Can you explain how long he can survive? i. without water and food? ii. without food but with water? 

Q 2. The first step in the detoxification of ethanol in the liver is its oxidation to form acetaldehyde. This reaction, catalyzed by alcohol dehydrogenase, produces large amounts of NADH. One common effect of alcohol intoxication is the accumulation of lactate in the blood. Can you explain why this effect occurs? 

 1. Comment “Fatty acids are storage fuels”. The weight of a person ABC is 65 kg. About 11.5 kg of his weight is due to the fat deposits in the body. He went for a hunger strike. Can you explain how long he can survive? i. without water and food? ii. without food but with water? 

Carbonic anhydrase is strongly inhibited by the drug acetazolamide, which is used as a diuretic. Carbonic anhydrase plays a significant role in the secretary processes because it participates in regulating the pH and bicarbonate of body fluids. The experimental curve of initial reaction velocity versus [S] for the carbonic anhydrase is shown below. When the experiment is repeated in the presence of acetazolamide, the lower curve is obtained. Determine the nature of inhibition. Explain with reason.

You have a 750 mL bottle of vodka containing 43 % (w/v) ethanol (MM = 46.07 g/mol = 0.789 g/ml). How many molecules of ethanol are in the bottle

Explain why gluconeogenesis is not the mere reversal of glycolysis. Give at least three reasons to support your answer.

Your answer must include the terms, endergonic, exergonic, reciprocal regulation and futile cycle.

Explain how giving an alcoholic patient who has hypoglycemia an IV infusion of glucose alone can be fatal.

Q3. The Trypsin-EDTA solution used in the experiment would contain 0.4% trypsin (w/v) and

0.03% EDTA (w/v). It is provided to you as a 10x stock solution.  

(a) EDTA has a molecular weight of 292.24. What is the molarity of the EDTA in the 10X stock solution? (3 marks)

(b) What is the total final amount (in mg) of Trypsin which is added to the cells? (2 marks) 

Q4. Following a 2-minute incubation, FBS solution is then added to the cells to deactivate the trypsin enzyme. The solution comprises 10% FBS, made up in 1x PBS. You decide to make up 50 ml of this solution using your 10 x PBS stock, and a 100% FBS stock.

(a) How much 10 x PBS will you need to use to make the 50 ml solution? (1 mark)

(b) How much 100% stock FBS would you need? (2 marks) 

I also need the workings out for the answers to these questions. Thank you.

 Q3. The Trypsin-EDTA solution used in the experiment would contain 0.4% trypsin (w/v) and

0.03% EDTA (w/v). It is provided to you as a 10x stock solution.  

(a) EDTA has a molecular weight of 292.24. What is the molarity of the EDTA in the 10X stock solution? (3 marks)

(b) What is the total final amount (in mg) of Trypsin which is added to the cells? (2 marks)