Biochemistry Answers

what is the relationship between d-altrose and d-glucose

What is the relationship between amino acids and the nitrogenous bases of purines and pyrimidines?

What is the relationship between amino acids and the nitrogenous bases of purines and pyrimidines?

Why a degenerate genetic code helps protect an organism from the effects of mutations?

what is a null and alternative hypothesis of the reduction of factories farming to decrease greenhouse gas emission?

what is a null and alternative hypothesis of how effective are engineering bacteriophages in treating bacteria

What are the different role of sugar neuclotides in the biosynthesis of glycogen? Briefly describe

Why fructose metabolised more rapidly than glucose ? Briefly describe about this.

any clue anyone knows how to answer any of these or attempt to?

Q3. The Trypsin-EDTA solution used in the experiment would contain 0.4% trypsin (w/v) and 

0.03% EDTA (w/v). It is provided to you as a 10x stock solution.   

(a)  EDTA has a molecular weight of 292.24. What is the molarity of the EDTA in the 10X stock solution? 

(b)  What is the total final amount (in mg) of Trypsin which is added to the cells?

Q4. Following a 2 minute incubation, FBS solution is then added to the cells to deactivate the trypsin enzyme. The solution comprises 10% FBS, made up in 1x PBS. You decide to make up 50 ml of this solution using your 10 x PBS stock, and a 100% FBS stock. 

(a) How much 10 x PBS will you need to use to make the 50 ml solution?

(b) How much 100% stock FBS would you need?

Glycogenolysis

1) What is glycogen? Where is it stored?

2) Glucagon, a hormone found in the pancreas, stimulates what enzyme found only in the liver and not in skeletal muscles? Identify the two pathways where this enzyme is used.

3) In the reaction: glycogen to glu-1-phosphate, what reaction is involved? Name the enzyme.

4) In the reaction: glu-1-phosphate to glu-6-phosphate, what reaction is involved? Name the enzyme. Identify the two pathways where this enzyme is used.

5) In the reaction: glu-6-phosphate to glucose. What reaction is involved? Name the enzyme. Identify the two pathways where this enzyme is used.

Glycogenesis

6) In the reaction: glucose to glu-6-phosphate, what reaction is involved? Name the enzyme. Identify the two pathways where this enzyme is used.