Biochemistry Answers

Feedback inhibition involves...

a)noncompetitive inhibitors

b)competitive inhibitors

c)cofactors

d)high concentration of substrate

e)low concentration of enzyme

Clinical manifestation of colles fracture deformity

purine nucleoside phosphorylase catalyzes a phosphorylytic cleavage of

compare and contrast the environment of the chicken and the environment of the t rex

Long discussion on Greenhouse gases and global warming

Briefly discuss stereoisomerism with reference to carbohydrates.  

which salts are associated with temporary water hardness?

Why are enzymes considered as biochemical markers?

Deoxyribonucleotide triphosphates (dNTPs: dATP, dTTP, dCTP and dGTP) are critical components in polymerase chain reactions (PCR) for the amplification of DNA fragments in the lab. Each dNTP is provided individually as a stock solution with a concentration 1 mM.

a)  What volume of each of the four dNTP stock solutions, and what volume of water must be mixed together to prepare 200 μL of solution A, in which the concentration of each dNTP is 0.1 mM (i.e. in solution A the concentration of the four dNTPs will be 0.1 mM dATP, 0.1 mM dTTP, 0.1 mM dCTP, 0.1 mM dGTP)? (3 points)

b)  0.1 μL of solution A were added in a PCR reaction with a final volume of 20 μL. What is the concentration of each individual dNTP in the PCR reaction? Give your answer in units of nM. (3 points)

What is the protein structure (primary, secondary tertiary or quaternary) of Elastin?