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Answer to Question #126646 in Molecular Biology for Brian

Question #126646

Describe the process of amplification and purification of λgt10 phage for cloning


1
Expert's answer
2020-08-11T12:02:19-0400

Phage particles are isolated by using precipitation with polyethylene glycol and its subsequent purification by centrifugation in CsCl step gradients and by equilibrium centrifugation. Amplification of specific phage is completes by primer specific PCR.


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