Biochemistry Answers

1. What is the significance of having no fat deposits between the skin and muscle for a frog in cold weather?

A 59yr old man, Koosei was admitted unconscious to the emergency room from a local bar days after DNA tests revealed he was not the biological father of his 6 children. The bartender revealed that he is able to eat only a half-ball of kenkey and black pepper and drinks lots of Oyaw Bitters. Lab result: Test Result Interpretation Blood alcohol 550mg/dl high Plasma Glucose 50mg/dl low Blood lactate 8mmol/L high Blood pH 7.21 low

(i) Give a diagnosis of the clinical condition based on the lab findings. (4mks)

(ii) State the biochemical basis for each of these four (4) results? (8mks)

(iii) Name two (2) biochemical pathways which will be affected in this condition? (4mks)

(iv) How will these pathways be affected? (4mks)

Given the overall equation for respiration (C₆H₁₂O₆ + 6O₂ 6CO₂ + 6H₂O), in which step(s) in the respiratory pathway is/are each of the reactants used up and each of the products made? 

Mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS) is a mitochondrial inherited disorder due to deficiency of either NADH/COQ reductase (Complex I cytochrome oxidase (Complex IV). Due to this lack of a functional mitochondria, what would the net ATP produced from one molecule of glucose? 6+2=8.

A. 0

B.1

2

D. 4

E. 8

Why would the pH of your blood plasma increase if you breathe fast?

Give an account of the total ATP yield when I molecule of glucose convert carbon dioxide and water

- Briefly explain why we incubate the purified plasmid with the restriction enzymes, BamHI and XhoI, at exactly 37oC. 

Assume the linear fit of the standard curve using DNSA absorbance at 540nm versus glucose concentration in M is y=0.002x + 0.003. Calculate the concentration of an unknown solution of glucose with DNSA absorbance at 540nm of 0.17.

What is the importance of nucleic acid studies in elucidation and treatment of diseases

State three errors of nucleic acid metabolism, note in each case the defective enzyme