Answer to Question #98375 in Cell Biology for Abhishek

Question

EXPLAIN SEMI - CONSERVATIVE REPLICATION OF DNA . WHAT ARE THE ENZYMES INVOLVED IN THIS PROCESS ? WRITE THEIR FUNCTIONS .

Answer 1

Semi-conservative replication creates two copies, each containing one of the original (parental) chains and one new (daughter).

Helicase splits the chains of a double stranded DNA molecule

Primase synthesizes a short RNA fragment, called a primer, complementary to a single-stranded DNA matrix

DNA-polymerase catalyzes polymerization of deoxyribonucleotides along the DNA nucleotide chain

Telomerase adds special repeating DNA nucleotide sequences to the 3'-end of the DNA strand at the telomere sites that are located at the ends of the chromosomes in eukaryotic cells.

Ligase catalyzes the connection of two molecules with the formation of a new chemical bond - ligation

Topoisomerase relaxes supercoiled DNA molecules by introducing single- or double-stranded breaks with subsequent restoration